Description
Recombinant Mouse CD14 is produced from mouse CD14 transfected CHO-cells in serum free medium and purified by TALON His-tag purification. Before transfection, the complete mouse CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. A DNA sequence encoding the extracellular domain (Met 1 - Pro 345) of mouse CD14 (NP_033971.1) precurser was fused with a polyhistidine tag at the C-terminus end